Abstract
The developmental process of goldfish is quite similar to that of zebrafish. This high similarity facilitates developmental biological studies in goldfish because methodologies that are frequently used in zebrafish may be easily adapted; for example, in situ hybridization and microinjection protocols can be adapted to goldfish. However, several goldfish-specific features still hinder experimental work in this species. Here, the accumulated knowledge in our laboratory provides the basis for detailed procedures for artificial fertilization, including obtaining fertilized eggs and experimental techniques like microscopic observations, histology, genotyping, analyses of the gene expression patterns, and functional analyses of genes. The techniques are illustrated with pictures, and I hope that this chapter will benefit enthusiastic breeders and fanciers who want to carefully observe the developmental process of their goldfish as well as all researchers who want to start goldfish evodevo studies in their own laboratories.
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Ota, K.G. (2021). Experimental Notes. In: Goldfish Development and Evolution. Springer, Singapore. https://doi.org/10.1007/978-981-16-0850-6_8
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DOI: https://doi.org/10.1007/978-981-16-0850-6_8
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