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Evaluation of Freshwater Mussel (Anodonta cygnea) Protein Hydrolysates in Terms of Antibacterial Activity and Functional Properties

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Abstract

Bioactive peptides from dietary proteins are used as nutraceuticals. The objective of the investigation is to produce enzymatically hydrolyzed bioactive peptides from the freshwater mussel Anodonta cygnea using commercial food-grade proteases (Alcalase® 2.4 L, and Pepsin). Antibacterial activity of protein hydrolysates against (Escherichia coli, Salmonella typhi, Bacillus subtilis, Staphylococcus aureus, Listeria monocytogenes), FTIR, molecular weight, functional characteristics (protein solubility at different pH, fat absorption, water holding capacities) were evaluated. The degree of hydrolysis increased in higher enzyme concentrations. Hydrolyzed mussel proteins could inhibit some bacterial growth. The hydrolyzed samples by 1 and 6% enzymes showed the highest oil absorption capacity and the 3% treatment had the highest water absorption capacity. By increasing the percentage of enzymes, the solubility of protein increased. The SDS-page results appeared bands less than 2 kD. Our results suggest that swan mussel hydrolysate may be useful as an antibacterial agent, but further studies are needed to exact hydrolysate conditions.

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Acknowledgements

This work has been partly supported by Iran National Science Foundation (INSF).

Funding

This study was funded by Iran National Science Foundation.

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Authors and Affiliations

  1. Department of Seafood Processing, Faculty of Fisheries and Environmental Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran

    Sakine Heydari, Parastoo Pourashouri & Bahareh Shabanpour

  2. Department of Medical Biotechnology, Faculty of Advanced Technologies in Medicine, Gorgan University of Medical Sciences, Gorgan, Iran

    Fatemeh T. Shamsabadi & Mehdi Sheikh Arabi

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  1. Sakine Heydari
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  2. Parastoo Pourashouri
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  3. Bahareh Shabanpour
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  4. Fatemeh T. Shamsabadi
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Correspondence to Parastoo Pourashouri.

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Heydari, S., Pourashouri, P., Shabanpour, B. et al. Evaluation of Freshwater Mussel (Anodonta cygnea) Protein Hydrolysates in Terms of Antibacterial Activity and Functional Properties. Waste Biomass Valor (2023). https://doi.org/10.1007/s12649-023-02301-6

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