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Mitogenome analysis of dwarf pufferfish (Carinotetraodon travancoricus) endemic to southwest India and its implications in the phylogeny of Tetraodontidae

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Abstract

The Tetraodontidae (pufferfishes), is primarily a family of marine and estuarine fishes with a limited number of freshwater species. Freshwater invasions can be observed in South America, Southeast Asia and central Africa. In the present study, we have analysed the complete mitogenome of freshwater pufferfish, Carinotetraodon travancoricus (dwarf pufferfish or Malabar pufferfish) endemic to southwest India. The genome is 16487 bp in length and consist of 13 protein-coding genes, 22 transfer RNA genes, two ribosomal RNA genes and one control region like all the other vertebrate mitogenomes. The protein-coding genes ranged from 165 bp (ATP synthase subunit 8) to 1812 bp (NADH dehydrogenase subunit 5) and comprised of 11310 bp in total, constituting 68.5% of the complete mitogenome. Some overlaps have been observed in protein-coding genes by a total of 7 bp. The AT skew (0.032166) and GC skew (−0.29746) of the mitogenome indicated that heavy strand consists equal amount of A and T, but the overall base composition was mainly C skewed. The noncoding D-loop region comprised 869 bp. The conserved motifs ATGTA and its complement TACAT associated with thermostable hairpin structure formation were identified in the control region. The phylogenetic analysis depicted a sister group relationship of C. travancoricus with euryhaline species Dichotomyctere nigroviridis and D. ocellatus with 100% bootstrap value rather than with the other freshwater members of Carinotetraodon species from Southeast Asia. The data from this study will be useful for proper identification, genetic differentiation, management and conservation of the dwarf Indian pufferfish.

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Acknowledgements

This work was supported by Centre of Excellence in Sustainable Aquaculture and Aquatic Health Management (CAAHM), Plan fund, Kerala University of Fisheries and Ocean Studies.

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Authors and Affiliations

  1. Department of Aquaculture, Kerala University of Fisheries and Ocean Studies, Panangad, 682 506, India

    Chandhini Sathyajith & Rejish Kumar Vattiringal Jayadradhan

  2. The University Museum, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-0033, Japan

    Yusuke Yamanoue

  3. School of Life Sciences, Tokyo University of Pharmacy and Life Sciences, 1432-1 Horinouchi, Hachioji, Tokyo, 192-0392, Japan

    Shin-Ichi Yokobori

  4. Planet Earth Aquarium, Mysuru, 571 606, India

    Sunesh Thampy

Authors
  1. Chandhini Sathyajith
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  2. Yusuke Yamanoue
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  3. Shin-Ichi Yokobori
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  4. Sunesh Thampy
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  5. Rejish Kumar Vattiringal Jayadradhan
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Correspondence to Rejish Kumar Vattiringal Jayadradhan.

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Corresponding editor: H. A. Ranganath

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Sathyajith, C., Yamanoue, Y., Yokobori, SI. et al. Mitogenome analysis of dwarf pufferfish (Carinotetraodon travancoricus) endemic to southwest India and its implications in the phylogeny of Tetraodontidae. J Genet 98, 105 (2019). https://doi.org/10.1007/s12041-019-1151-9

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  • DOI: https://doi.org/10.1007/s12041-019-1151-9

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