Abstract
Swarming locusts cause huge plagues across the world threatening food production. Before swarms form, locust populations exhibit a dramatic phase change from a solitary to a gregarious phase. The cause of this phase change is a complicated interplay of conspecific and environmental cues and is, especially for one of the major pests, the migratory locust Locusta migratoria, still not well understood. Here we study the behavior of both solitary and gregarious L. migratoria towards the headspace odors of conspecifics. As we do not find a general attraction of gregarious animals to the headspace of gregarious conspecifics, swarm formation does not seem to be mainly governed by olfactory aggregation cues. When testing for potential mating signals, we observe that the headspace of virgin gregarious females is highly attractive only towards virgin males of the same phase, while mated gregarious males and solitary males, regardless of their mating state, do not become attracted. Interestingly, this phase-specific attraction goes along with the finding, that mating behavior in experiments with inter-phasic pairings is extremely rare. Our data suggest that odor emissions in L. migratoria play a significant role in a mating context.
Similar content being viewed by others
Introduction
The devastating effect of swarms of gregarious locusts has been reported since biblical times. While solitary and gregarious locusts had been considered to be different species, in 1921, Uvarov discovered the fascinating phenomenon of phase polyphenism (Pflüger and Bräunig 2021; Simpson et al. 2011). As we now know from several locust species (Topaz et al. 2012), Locusta migratoria (Linnaeus 1758; Order: Orthoptera, Family: Acrididae) exists in solitary and gregarious phases. These phases differ in morphological, anatomical, and behavioral features (Greenwood and Chapman 1984; Latchininsky 2019; Wang et al. 2014; Wei et al. 2017), with e.g., the solitary phase being more camouflaged, while the gregarious phase is conspicuously colored. The shift between both phases is a complex phenomenon, that to some extent is still considered a puzzle. In desert locusts Schistocerca gregaria (Forsskål 1775; Order: Orthoptera, Family: Acrididae), factors such as environmental cues and sensory cues from conspecifics, including visual, tactile, and olfactory cues, seem to be involved (Nakano et al. 2022; Roessingh et al. 1998; Simpson et al. 2011). The role of olfaction in the locusts’ phase polyphenism has been explored over the past few decades (Guo et al. 2020; Wei et al. 2017). It is well understood that odor profiles are dynamic within the life stages and between the phases (Wei et al. 2017). Most of the existing literature, however, focuses on S. gregaria and suggests that headspace odors from gregarious animals are attractive to gregarious and repulsive to solitary conspecifics (Roessingh et al. 1993, 1998; Rogers et al. 2003). However, as S. gregaria and L. migratoria even differ in their responses to the pivotal body odor phenylacetonitrile (PAN) that is present in both species (Pener and Simpson 2009; Torto et al. 1996; Wei et al. 2019), the general function of headspace odors in L. migratoria so far remains elusive. Moreover, phase shift dynamics also differ directionally between the phases in S. gregaria (Simpson et al. 1999). However, there is a lack of understanding about L. migratoria that prompts further research to understand the behavioral response of L. migratoria to conspecific smells.
Here, we provide headspace odor collections of different developmental stages of gregarious L. migratoria to individual animals in a binary choice arena. Some studies have so far focused on the behavioral responses towards animal-released odors mainly in S. gregaria (Obeng-Ofori et al. 1993; Torto et al. 1996). We, therefore, performed a comprehensive set of experiments to test, whether stage-, phase-, and/or sex-specific odor blends provoke attraction or repulsion to the different stages, phases, and sexes in L. migratoria. At the same time, interphasic mating occurs in S. gregaria (Golov et al. 2018a) but this phenomenon has not been studied in L. migratoria. We, therefore, extend our study to mating assays between the different phases of L. migratoria. In conclusion, we aim to increase our understanding of L. migratoria with regard to the behavioral impact of its body odors.
Methods and Materials
Animal Breeding
We used L. migratoria that we bought from a local pet shop. The gregarious and solitary animals used for tests were kept separated for a minimum of 5 generations. Both phases were maintained at the Max Planck Institute for Chemical Ecology in climate chambers with a 14:10 h light:dark cycle, at a temperature of 30 ± 2 °C, and humidity of 50 ± 5%. The gregarious animals were kept with around 300–400 first instar animals in a cubic cage (side length, 30 cm × 30 cm × 30 cm). The numbers of animals were reduced continuously during aging, to finally reach around 200 adult animals in the same cage. The solitary animals were separated on the day of hatching into individual cylindrical boxes (height, 10.5 cm; diameter 8 cm). Each solitary animal was supplied with a separate ventilation system. Both phases were fed with wheat grass provided by our greenhouse.
Bioassay Arena
The behavioral setup includes a cuboid base, an arena surface consisting of a mesh, and an arena enclosure (Fig. 1a). The base of the behavioral setup consists of two separate polypropylene boxes (A) (16 cm × 30.5 cm × 25 cm) with air diffusers opening upwards at the middle of the lower surface (B) of each of the boxes. The diffuser is connected to the odor/control source (C). The base is high enough to evenly distribute the odor at the base before the air enters the arena. The in-house air is controlled by two flowmeters connected to the odor and control source via a 6/4 mm Teflon pipe. The air inlet is kept at 3L/min for each side. From the source, the air is introduced to the base of each side of the setup through the diffuser. Two perforated polypropylene plates of size 25 cm × 30 cm, with perforations of 2 mm diameter and distributed evenly at every 2 mm are placed on each of the two boxes making a rectangular behavioral arena (D). The arena has no division in the middle, allowing the locust to move in all directions within the arena. This design results in an arena divided into two zones, one with odor and one without that the animal can chose depending on the valence of the tested odor. The arena is enclosed by another rectangular polypropylene box (26 cm × 62 cm × 39 cm) to limit the locust within the arena (E). A circular inlet of 5 cm diameter that can be opened and closed from outside is situated at the same level as the behavioral arena plane to introduce locusts into the arena (F). A rectangular opening of 29 cm × 25 cm with a closing door is situated above the animal inlet to retrieve the animal after each trial, with the least disturbance to the airflow (G). The base, arena, and enclosing walls are supported with aluminum hinges of 1 cm width. A pair of axial fans (H) (connected to the same voltage input) are suspended on top of each side 39 cm above the arena to ensure a laminar and vertical airflow (ca. 3 cm/s) in the arena. Between the fans, an HD USB camera covering the whole arena is fitted and connected to a computer outside. The whole setup is placed inside an enclosing chamber 92*72*65 cm with solid white walls to exclude visual distraction from outside of the arena (I). LED light is fitted 11 cm above the enclosure to ensure even lighting throughout the arena surface. The air from the enclosure is ventilated out at a constant rate of approximately 30L/min via an exhaust fitted 15 cm above the behavioral setup. The setup is maintained at 31 ± 1°C and humidity at 50% during the trials.
Bioassay Procedure
For each trial, one animal was introduced into the arena through the animal inlet, and each animal was tested only once. If an animal refused to enter the arena within the first 5 min, it was removed, and a new trial was started with a new animal. The 10-min recording of the animal’s behavior started only after the animal made an initial movement of at least 1 cm or more into the arena. After every 5 trials, the arena was wiped with 70% ethanol and ventilated for an hour with clean air to remove any odors and any potential trails left by the animals.
20 unstarved animals in an air-tight box were used as an odor source. After every 5 trials, the animals used as the odor source were placed back into the breeding cage for an hour after which they were again used as the odor source in the setup. In all tests, the side of odor within the arena was reversed after half of the trials. The nymphs used were all in the late fourth instar and not separated by males or females. The virgin animals for experiments were taken 6–8 days post eclosion and the mated animals were taken a few hours after the male debarks the female or the female finishes oviposition. The solitary animals were simply marked on the cage on the date of eclosion and used 8 days later for the tests.
Mating Experiments
To measure the willingness to mate, we used mounting behavior by males (virgin animals 6–8 days after the final eclosion) as a parameter. The animals were kept together in the cage and were observed until the first mounting happened. The mounted pair was removed and the remaining animals were separated into males and females and used for experiments within 48 h of separation. Mating tests were done in a 10 cm × 10 cm cage built from perforated plates on 5 sides and a glass on one side allowing observation. Each cage, considered as one data point, consists of four males and five females. The proportion of the number of males mounting the females was observed at 6 and 12 h. This was done inter-phase as a test and intra-phase as control experiments.
Data Analysis
In the odor choice bioassay procedure, the time (in seconds) an individual animal spent in the control or the odor side was observed. We considered the exact middle of the setup to be the division and the place of the head of the animal as the animal’s location when the animal spent time in the 2 cm-wide fringe area of the two zones.
The preference index was calculated as: \(PI=(Time in Odor(s)-Time in Control(s))/(Total time (600s))\). To test whether the preference was significantly attractive or aversive, we used the Wilcoxon-signed rank test.
Mounting behavior was analyzed by the Mann Whitney-U test, at 6 h. To compare between multiple groups, at 12 h, Kruskal–Wallis test with Dunn’s posthoc test for multiple comparisons was used. In all cases, both for PI and mating experiments, ***, p < 0.001; **, p < 0.01; *, p < 0.05. Wilcoxon-signed rank test was performed in R version 4.2.3. For multiple group comparison GraphPad InStat was used.
PAN used for the experiments was ordered in the highest purity available from Sigma Aldrich (B19401-250G).
Results
Evaluation of Bioassay
To screen for the behavioral valence of body odors, we used a two-zone arena, where the headspace of 20 animals from a given stage was infused to one side, while the other side was infused with control air (Fig. 1a). The time spent by individual animals in either zone was measured to investigate whether the odor was perceived as attractive (i.e., more time spent on the odor side), or repellent (i.e., more time spent on the control side).
To test the assay for functionality, experiments were first performed with an attractive food odor (i.e., headspace emitted by 5 g of shredded wheat grass) and with the known repellent PAN (100 µL at 1 mg/mL concentration) diluted with mineral oil. PAN is repulsive in this assay to all stages and phase of the L. migratoria (Chang et al. 2023). When testing starved fourth instar gregarious nymphs with the food odor, the animals showed strong attraction to the odor and spent significantly more time on the side of the arena smelling of wheat (Fig. 1b). Animals of the same cohort tested with PAN avoided the side with this odor (Fig. 1b), demonstrating that the assay indeed was suitable for testing both attraction and aversion.
Responses of Nymphs in Bioassay
Gregarious locusts usually aggregate in conspicuous huge hopper bands or swarms, whereas locusts of the solitary phase avoid groups and are rather cryptic. To investigate whether the aggregation and repulsion in gregarious and solitary animals, respectively, is governed by olfactory cues from the gregarious nymphs, the valence of odors from gregarious nymphs was tested in gregarious and solitary nymphs. Surprisingly, we did not find any significant response either in gregarious or in solitary nymphs to the headspace of gregarious nymphs (Fig. 2a). Similarly, no other tested headspaces from gregarious adults, except for the headspace of gregarious virgin females, elicited any significant attraction in gregarious or solitary nymphs (Supplementary Fig. 1). It thus seems that olfactory cues alone are not sufficient for the forming of hopper bands in gregarious L. migratoria.
Responses of Adults in Bioassay
We next asked whether attraction towards potential mates might be governed by olfactory cues. When testing gregarious virgin males and virgin females with the headspace of the opposite sex, males were significantly attracted to the female odor, while females did not respond to the male odor (Fig. 2b, c). Interestingly, when testing for inter-phase attraction, we found that the odors from gregarious virgin females did not elicit any response in solitary virgin males (Fig. 2b). In addition, contrary to the headspace of virgin gregarious females, the odor emitted by mated gregarious females was not attractive to virgin gregarious males (Fig. 2d), suggesting that the headspace of mated females is either lacking attractive compounds or includes repellent compounds that virgin females are not emitting. At the same time, the attraction of gregarious males towards the odor of virgin gregarious females was diminished when the males were already mated (Fig. 2c).
Mating Experiments
Having found that solitary males showed no attraction to the headspace of virgin gregarious females, we tested if this results in an inter-phase mating barrier. We conducted mating experiments, where virgin males were paired with either virgin females of the same or opposite phase (Fig. 3a). We then observed the mounting behavior which is an obligatory and easily observable step in orthopteran mating. Interestingly, we found that the frequency of mounting was significantly lower in inter-phase tests. At the end of 6 h, while 59% of the gregarious males mounted gregarious females, only 10% of the solitary males mounted the gregarious females. At the same time, 54% of the solitary males mounted the solitary females, while none of the gregarious males mounted solitary females during the 6 h of observations (Fig. 3b). While intra-phase combinations resulted in similar mounting ratios in gregarious and solitary animals during the first 6 h of the experiments, the mounting behavior of gregarious animals lasted longer, as many solitary males had already unmounted their solitary females by the end of 12 h (Fig. 3b).
Discussion
The phase state in locusts has been shown to be governed both by environmental cues and cues from conspecifics (Nakano et al. 2022). From a sensory point of view, different developmental stages of S. gregaria aggregate based on both chemo- and mechanosensory cues (Niassy et al. 1999; Rogers et al. 2003; Simpson et al. 2001). Contact cues and short-range odors seem to be pivotal in the gregarious phase, while long range auditory cues are more important in the solitary phase (Nakano et al. 2022; Pener and Simpson 2009). The short-range odors emitted and perceived by the gregarious animals emanate from food, feces, and the headspace of conspecifics. Based on these results we expected, that gregarious nymphs of L. migratoria, which form hopper bands, are attracted by the headspace of gregarious nymphs. Surprisingly, we did not find any attraction, and gregarious nymphs in most aspects did not differ from solitary ones, which were neither attracted nor repulsed by any of the gregarious headspaces.
S. gregaria have been reported to shift easily from the solitary to the gregarious phase (Rogers et al. 2003), whereas for L. migratoria, the solitary phase seems to be the more stable state, and a shift to the gregarious phase is difficult to induce and needs an extremely high density of animals. Corresponding to that the rate of gregarization and solitarisation is also different within and between locust species (Topaz et al. 2012). S. gregaria exhibit a much faster gregarisation and a slower solitarisation process (Simpson et al 1999; Wei et al. 2017). A higher concentration of odors or even a combination of multi-modal cues including e.g., mechanosensory or visual signals might thus potentially be necessary to trigger aggregation behavior among L. migratoria nymphs.
The headspace composition has been shown to be dynamic and dependent on phase and state (Wei et al. 2017). 4-vinylanisole (4 VA) is one of the headspace odors that has been identified as a possible aggregation pheromone in L. migratoria (Guo et al. 2020). PAN is another dominant headspace odor in both L. migratoria and S. gregaria. The role of PAN in S. gregaria is still under debate, as some studies suggest a role as an aggregation pheromone (Torto et al. 1994), others supposed repellency (Seidelmann et al. 2005), while yet others even suggest a role in sexual behavior (Seidelmann and Ferenz 2002). In L. migratoria, however, PAN is repulsive to animals of all stages and phases (Chang et al. 2023; Wei et al. 2019). The responses of nymphs that we observed in the aggregation tests could thus be expected, considering the odor profile of individual groups. The higher proportion of PAN to 4VA in gregarious males as compared to gregarious females (Wei et al 2019) could form a background to the neutral response to gregarious male odors, where the attractant 4VA and the repellent PAN balance each other. The strongly attractive response by gregarious nymphs to gregarious females could rely on the higher amount of 4VA, which would dominate over the repellency of PAN. Similar mixture interactions in binary mixtures of opposing valence have also been reported in e.g., Drosophila (Mohamed et al. 2019; Thoma et al. 2014).
When looking for potential effects of headspace odors on mating behavior, we found a strong attraction of gregarious males to the headspace of virgin gregarious females. As the number of gregarious males is much higher than that of gregarious females in mating swarms and in ovipositing populations (Ellis and Ashall 1957), gregarious males face strong competition for females. The indifferent response of the virgin gregarious females to the headspace of virgin gregarious males is coherent with this proportion, as females do not have to actively respond to males for successful mating. The lack of response of virgin gregarious males to mated females could indicate the presence of a courtship inhibitory factor. Such a factor can be either produced by the female herself to prevent further harassment from males (Engel et al. 2016) or be transferred by the male during mating as an additional passive form of mate guarding (Seidelmann 2006). Male-transferred anti-aphrodisiacs are found in other insects too (Khallaf et al. 2020, vander Meer et al. 1986). In S. gregaria, PAN acts as such a courtship inhibition pheromone (Seidelmann and Ferenz 2002). Alternatively, mated females could emit lower amounts of pheromones. The lack of response by mated gregarious males to the odor of virgin gregarious females could be interpreted as a sign of transient abstinence during a recovery phase (Barrozo et al. 2010a) which has been shown in other insects to be combined with a lower sensitivity to female pheromones post mating (Barrozo et al. 2010b).
When testing mating behavior within the phases, we already found differences between the gregarious and the solitary animals. The mounting of solitary males usually lasted for less than 12 h, while that of gregarious males lasted much longer. Mounting of females by males is the longest copulation step and is present in both phases. It has been proposed as an active mate guarding strategy of the male to avoid remating of the female before his sperm has fertilized her eggs (Golov et al. 2018a; Zhu and Tanaka 2002). The duration of the mounting varies depending on sub species, duration of separation of males into cohorts, and phase (Golov et al. 2018a, b; Seidelmann 2006; Tanaka and Zhu 2003). The longer mounting that we observed in the gregarious phase could, hence, be interpreted as an effect of the higher male-male competition faced by gregarious males within the swarm.
In the inter-phase attraction experiments, we found it intriguing that solitary males were not attracted by the headspace of virgin gregarious females. We, therefore, asked whether males and females from different phases would mate at all. In S. gregaria gregarious males exhibit frequent mounting attempts when encountering solitary females. Interestingly, we found an opposite trend in L. migratoria. Males of a given phase showed only weak interest in females of the other.
In conclusion, we found that odors emitted by nymphs seem to be of less importance in attracting other nymphs into hopper bands. Other sensory cues or odors of a higher concentration, or combinations of these might be the deciding factors. The attraction between the sexes does, however, seem to be relying on female-produced cues as gregarious males were strongly attracted to the odor of virgin gregarious females. Interestingly, solitary males were not attracted to the smell of gregarious females, revealing a certain degree of an inter-phase mating barrier. This postulation was further corroborated by our finding that male mounting behavior in couples of mixed phases is very rare.
Data Availability
Data will be made available on request.
Change history
15 March 2024
A Correction to this paper has been published: https://doi.org/10.1007/s10886-024-01487-w
References
Barrozo RB, Gadenne C, Anton S (2010a) Post-mating sexual abstinence in a male moth. Commun Integr Biol 3:629–630
Barrozo RB, Gadenne C, Anton S (2010b) Switching attraction to inhibition: mating-induced reversed role of sex pheromone in an insect. J Exp Biol 213:2933–2939
Chang H, Cassau S, Krieger J et al (2023) A chemical defense deters cannibalism in migratory locusts. Science 380:537–543
Ellis PE, Ashall C (1957) Field studies on diurnal behaviour, movement and aggregation in the desert locust (Schistocerca gregaria Forskål). Anti-Locust Bull 25:4–94
Engel KC, Stökl J, Schweizer R et al (2016) A hormone-related female anti-aphrodisiac signals temporary infertility and causes sexual abstinence to synchronize parental care. Nat Commun 7:1–10
Forsskål P (1775) Descriptiones animalium, avium, amphibiorum, piscium, insectorum, vermium; quae in itinere orientali observavit Petrus Forsskål. Post mortem auctoris edidit Carsten Niebuhr. Havenai, Mölleri, p 164
Golov Y, Harari A, Rillich J, Ayali A (2018a) Precopulatory behavior and sexual conflict in the desert locust. PeerJ 6:e4356
Golov Y, Rillich J, Douek M et al (2018b) Sexual behavior of the desert locust during intra-and inter-phase interactions. J Insect Behav 31:629–641
Greenwood M, Chapman RF (1984) Differences in numbers of sensilla on the antennae of solitarious and gregarious Locusta migratoria L. (Orthoptera: Acrididae). Int J Insect Morphol Embryol 13:295–301
Guo X, Yu Q, Chen D et al (2020) 4-Vinylanisole is an aggregation pheromone in locusts. Nature 584:584–588
Khallaf MA, Auer TO, Grabe V, Depetris-Chauvin A, Ammagarahalli B, Zhang DD, Lavista-Llanos S, Kaftan F, Weißflog J, Matzkin LM, Rollmann SM, Löfstedt C, Svatoš A, Dweck HKM, Sachse S, Benton R, Hansson BS, Knaden M (2020) Mate discrimination among subspecies through a conserved olfactory pathway. Sci Adv 6:25
Latchininsky AV (2019) Locusts. Encycl Anim Behav 18:115–127
Linnaeus C (1758) Systema naturae per regna tria naturae, secundum classes, ordines, genera, species, cum characteribus, differentiis, synonymis, locis. Tomus I. Editio decima, reformata 1:824
Mohamed AAM, Retzke T, Das Chakraborty S et al (2019) Odor mixtures of opposing valence unveil inter-glomerular crosstalk in the Drosophila antennal lobe. Nat Comm 10:1
Nakano M, Morgan-Richards M, Trewick SA, Clavijo-McCormick A (2022) Chemical ecology and olfaction in short-horned grasshoppers (Orthoptera: Acrididae). J Chem Ecol 48:121–140
Niassy A, Torto B, Njagi PGN et al (1999) Intra- and interspecific aggregation responses of Locusta migratoria migratorioides and Schistocerca gregaria and a comparison of their pheromone emissions. J Chem Ecol 25:1029–1042
Obeng-Ofori D, Torto B, Hassanali A (1993) Evidence for mediation of two releaser pheromones in the aggregation behavior of the gregarious desert locust, Schistocerca gregaria (forskal) (Orthoptera: Acrididae). J Chem Ecol 19:1665–1676
Pener MP, Simpson SJ (2009) Locust phase polyphenism: An update. Adv Insect Phys 36:1–272
Pflüger HJ, Bräunig P (2021) One hundred years of phase polymorphism research in locusts. J Comp Physiol A 207:321–326
Roessingh P, Simpson SJ, James S (1993) Analysis of phase-related changes in behaviour of desert locust nymphs. Proc Royal Soc B 252:43–49
Roessingh P, Bouaïchi A, Simpson SJ (1998) Effects of sensory stimuli on the behavioural phase state of the desert locust, Schistocerca gregaria. J Insect Physiol 44:883–893
Rogers SM, Matheson T, Despland E et al (2003) Mechanosensory-induced behavioural gregarization in the desert locust Schistocerca gregaria. J Exp Biol 206:3991–4002
Seidelmann K (2006) The courtship-inhibiting pheromone is ignored by female-deprived gregarious desert locust males. Biol Lett 2:525–527
Seidelmann K, Ferenz HJ (2002) Courtship inhibition pheromone in desert locusts, Schistocerca gregaria. J Insect Physiol 48:991–996
Seidelmann K, Warnstorff K, Ferenz H-J (2005) Phenylacetonitrile is a male specific repellent in gregarious desert locusts, Schistocerca gregaria. Chemoecology 15:37–43
Simpson SJ, McCaffery A, Hägele BF (1999) A behavioural analysis of phase change in the desert locust. Biol Rev 74:461–480
Simpson SJ, Despland E, Hägele BF, Dodgson T (2001) Gregarious behavior in desert locusts is evoked by touching their back legs. Proc Natl Acad Sci USA 98:3895–3897
Simpson SJ, Sword GA, Lo N (2011) Polyphenism in insects. Curr Biol 21:738–749
Tanaka S, Zhu DH (2003) Phase-related differences in mating strategy of a locust (Orthoptera: Acrididae). Ann Entomol Soc Am 96:498–502
Thoma M, Hansson BS, Knaden M (2014) Compound valence is conserved in binary odor mixtures in Drosophila melanogaster. J Exp Biol 217:3645–3655
Topaz CM, D’Orsogna MR, Edelstein-Keshet L, Bernoff AJ (2012) Locust dynamics: Behavioral phase change and swarming. PLoS Comput Biol 8:e1002642
Torto B, Obeng-Ofori D, Njagi PGN et al (1994) Aggregation pheromone system of adult gregarious desert locust Schistocerca gregaria (forskal). J Chem Ecol 20:1749–1762
Torto B, Njagi PGN, Hassanali A, Amiani H (1996) Aggregation pheromone system of nymphal gregarious desert locust, Schistocerca gregaria (Forskål). J Chem Ecol 22:2273–2281
vander Meer RK, Obin MS, Zawistowski S et al (1986) A reevaluation of the role of cis-vaccenyl acetate, cis-vaccenol and esterase 6 in the regulation of mated female sexual attractiveness in Drosophila melanogaster. J Insect Physiol 32:681–686
Wang X, Fang X, Yang P et al (2014) The locust genome provides insight into swarm formation and long-distance flight. Nat Comm 5:2957
Wei J, Shao W, Wang X et al (2017) Composition and emission dynamics of migratory locust volatiles in response to changes in developmental stages and population density. Insect Sci 24:60–72
Wei J, Shao W, Cao M et al (2019) Phenylacetonitrile in locusts facilitates an antipredator defense by acting as an olfactory aposematic signal and cyanide precursor. Sci Adv 5:5495–5518
Zhu DH, Tanaka S (2002) Prolonged precopulatory mounting increases the length of copulation and sperm precedence in Locusta migratoria (Orthoptera: Acrididae). Ann Entomol Soc Am 95:370–373
Funding
Open Access funding enabled and organized by Projekt DEAL. The study was funded by the Max-Planck Institute.
Author information
Authors and Affiliations
Contributions
All authors were involved in the design of the study; APU performed the experiments; APU and MK analyzed the data; APU and MK wrote the first draft of the manuscript; all authors contributed to the final version of the manuscript.
Corresponding authors
Ethics declarations
Ethical Approval
Not applicable.
Competing Interests
The authors have no competing interests to declare that are relevant to the content of this article. The authors have no relevant financial or non-financial interests to disclose.
Additional information
An article note shall be added: “The published online proof of this paper is incorrect and not the updated one as it still contains the watermark “Uncorrected Proof”.”
Markus Knaden and Bill S. Hansson share senior authorship.
Supplementary Information
Below is the link to the electronic supplementary material.
Rights and permissions
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
About this article
Cite this article
Unni, A.P., Knaden, M. & Hansson, B.S. Olfactory Mating Signals in the Migratory Locust Locusta migratoria. J Chem Ecol 50, 11–17 (2024). https://doi.org/10.1007/s10886-023-01456-9
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10886-023-01456-9