Abstract
Oryzias loxolepis, new species, is described from Lake Towuti in the Malili Lake system, central Sulawesi, Indonesia. Historically this new species has been confused with Oryzias marmoratus (Aurich 1935), which we redescribe. While both species share dark brown blotches on a grayish-brown trunk, O. loxolepis differs from O. marmoratus in having 11 abdominal vertebrae, 12 or 13 transverse scales, a shorter caudal peduncle, terminal mouth, reduced nuchal concavity, slanted trunk scales, and a rounded male dorsal fin. Oryzias loxolepis can be also distinguished from Oryzias profundicola Kottelat 1990, another sympatric congener in Lake Towuti, in having a longer snout in both sexes, in males in having a larger head and eyes, and a black margined dorsal fin, and in females in having a shallower body depth at the anal- and dorsal-fin origins. A phylogenetic analysis based on genome-wide single nucleotide variants reveals O. loxolepis to be a sister taxon to O. profundicola rather than O. marmoratus. The identities of specimens historically referred to “O. marmoratus” in previous genetic studies are re-evaluated.
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Acknowledgments
We thank I.F. Mandagi (Sam Ratulangi Univ.), B. Soeroto (Sam Ratulangi Univ.), S.A. Lawelle (Halu Oleo Univ.), K. Mochida (Nagasaki Inst. Appl. Sci.), J. Montenegro (JAMSTEC), R. Kakioka (Univ. Ryukyus), S. Ansai (Tohoku Univ.), and local villagers in Timampu (Bapak-Ryan and his families) for assistance with our field collections; H.H. Tan (ZRC) for providing radiographs; H.H. Tan and M. Kottelat (ZRC) for providing valuable comments for our manuscript; R. Kakioka for help and valuable comments for our phylogenomic analysis; Y. Takehana (Nagahama Inst. Bio.) for providing mitochondrial sequences; N. Hashimoto (freelance photographer) and K. Mochida for providing live photographs; M. Sato (NSMT) providing equipments for fluorescent staining of neuromasts; H.H. Tan, K.P. Lim, and P.K.L. Ng (ZRC), T. Naruse (Univ. Ryukyus), I.V. Utama (Univ. Ryukyus, MZB), R.K. Hadiaty (MZB), E. Dondorp (Naturalis), J. McLane (BMNH), K. Maeda (Okinawa Inst. Sci. Technol.), H. Senou (KPM), M. Nakae, H. Hata, and G. Shinohara (NSMT) for access to museum collections; T. von Rintelen (ZMB) for providing the original map. We also thank S. O’Shea (Edanz Co. Ltd.) for editing a draft of this manuscript. We are grateful the Ministry of Research, Technology, and Higher Education, Republic of Indonesia (RISTEKDIKTI) and the Faculty of Fisheries and Marine Science, Sam Ratulangi University, for the permit to conduct research in Sulawesi (research permit numbers 107/SIP/FRP/E5/Dit.KI/IV/2018). All experiments were approved by the Animal Care Committee of the University of the Ryukyus (201899). This study was supported by JSPS KAKENHI to HK (19J22686) and KY (17H01675 and 21H04782).
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Kobayashi, H., Mokodongan, D.F., Horoiwa, M. et al. A new lacustrine ricefish from central Sulawesi, with a redescription of Oryzias marmoratus (Teleostei: Adrianichthyidae). Ichthyol Res 70, 490–514 (2023). https://doi.org/10.1007/s10228-023-00908-2
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DOI: https://doi.org/10.1007/s10228-023-00908-2