Abstract
Species belonging to the Alismataceae (Echinodorus) and Limnocharitaceae (Hydrocleys and Limnocharis) families were analysed by banding with CMA/DAPI fluorochromes, C/CMA/DAPI banding, and in situ hybridization (FISH) with probes that recognise 45S rDNA. All species of Echinodorus presented 2n = 22, but only in E. lanceolatus were DAPI+ telomeric bands in seven chromosome pairs observed. A bimodal karyotype and GC-rich heterochromatin preferably located in two smaller acrocentric pairs that generally corresponded to the number of sites of 45S rDNA. A similar pattern of bands was observed in both Limnocharis species (2n = 20), but the two differed with respect to 45S rDNA, with L. laforestii showing only two sites. Hydrocleys nymphoides and H. martii had a chromosome number of 2n = 16, but the position of the GC-rich heterochromatin associated with the satellite differed among chromosomal types. In this work, the cytotaxonomic implications of these patterns are discussed and correlated with previous data from the literature.
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Acknowledgments
We thank the CNPq for the fellowship award to the first author, Prof. Marcelo Guerra of the Botany Department of Federal University of Pernambuco for allowing the use of the photomicroscope and TROPEN Institute (Núcleo de Referência em Ciências Ambientais do Trópico Ecotonal do Nordeste) for providing plant material and financial support.
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Feitoza, L.L., Martins, M.I.G., Castro, A.A.J.F. et al. Cytogenetics of Alismataceae and Limnocharitaceae: CMA/DAPI banding and 45S rDNA sites. Plant Syst Evol 286, 199–208 (2010). https://doi.org/10.1007/s00606-010-0300-7
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DOI: https://doi.org/10.1007/s00606-010-0300-7