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PC-KLH (Keyhole Limpet Hemocyanin) - chemical structure image

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PC-KLH (Keyhole Limpet Hemocyanin) - chemical structure image
PC-KLH (Keyhole Limpet Hemocyanin): sc-396490

PC-KLH (Keyhole Limpet Hemocyanin)

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Alternate Names:
Phosphorylcholine Keyhole Limpet Hemocyanin
Application:
PC-KLH (Keyhole Limpet Hemocyanin) is A PC hapten conjugated to KLH protein by p-DPPC
Molecular Formula:
C11H17N3O4P-KLH
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data.

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PC-KLH is a phosphorylcholine hapten conjugated to KLH protein by use of p-diazonium phenylphosphorylcholine.

References:

  1. Molecular analysis of the autoantibody response in peptide-induced autoimmunity.  |  Putterman, C., et al. 2000. J Immunol. 164: 2542-9. PMID: 10679092
  2. Analysis of a T cell receptor gene as a target of the somatic hypermutation mechanism.  |  Hackett, J., et al. 1992. J Exp Med. 176: 225-31. PMID: 1613457
  3. Identification of a B-1 B cell-specified progenitor.  |  Montecino-Rodriguez, E., et al. 2006. Nat Immunol. 7: 293-301. PMID: 16429139
  4. IgA attenuates anaphylaxis and subsequent immune responses in mice: possible application of IgA to vaccines.  |  Yamaki, K., et al. 2014. Immunol Res. 58: 106-17. PMID: 24366664
  5. Dickkopf-3 acts as a modulator of B cell fate and function.  |  Ludwig, J., et al. 2015. J Immunol. 194: 2624-34. PMID: 25672757
  6. Reduced Atherosclerosis in apoE-inhibitory Fc gamma RIIb-Deficient Mice Is Associated With Increased Anti-Inflammatory Responses by T Cells and Macrophages.  |  Ng, HP., et al. 2015. Arterioscler Thromb Vasc Biol. 35: 1101-12. PMID: 25792447
  7. Dendritic cell-targeting DNA-based nasal adjuvants for protective mucosal immunity to Streptococcus pneumoniae.  |  Kataoka, K., et al. 2017. Microbiol Immunol. 61: 195-205. PMID: 28463465
  8. Intranasal immunization with phosphorylcholine suppresses allergic rhinitis in mice.  |  Miyashita, K., et al. 2018. Laryngoscope. 128: E234-E240. PMID: 29193138
  9. Phosphorylcholine-Primed Dendritic Cells Aggravate the Development of Atherosclerosis in ApoE-/- Mice.  |  Dong, Q., et al. 2021. Circ Rep. 3: 86-94. PMID: 33693294
  10. Non-specific binding of mouse IgM antibodies to lipid antigens.  |  Hofstetter, W., et al. 1985. J Neuroimmunol. 7: 207-14. PMID: 3965496
  11. Immunologic memory to phosphorylcholine III. IgM includes a fine specificity population distinct from TEPC 15.  |  Chang, SP., et al. 1982. J Immunol. 129: 1559-62. PMID: 6980944
  12. Effect of age on the expressed B cell repertoire: role of B cell subsets.  |  Hu, A., et al. 1993. Int Immunol. 5: 1035-9. PMID: 7694639
  13. Mechanistic and selective constraints act on the establishment of V lambda J lambda junctions in the B cell repertoire.  |  Boudinot, P., et al. 1994. J Immunol. 152: 2248-55. PMID: 8133038

I tried to dissolve it into PBS as 1mg/mL, but it did not completely dissolved. Not sure what's happened?

Asked by: Jiansheng
Thank you for your question. This product can be dissolved in PBS buffer, water or other neutral buffers at a concentration of 1mg/mL. Here is the recommended PBS formulation: 8 g NaCl 0.2 g KCl 0.2 g potassium di-hydrogen phosphate (KH2PO4) 1.15 g di-sodium hydrogen phosphate (Na2HPO4) , add to 1 L of water.
Answered by: Tech Service
Date published: 2020-12-27

Hi, I am puzzled about the chemistry of PC-KLH, and how you make it. The formulas and its name are for a phosphorylcholine conjugate. What is the agent p-DPPC that is used? Is this dipalmitoylphosphatidylcholine? Is PC phospholipid?

Asked by: RobinP
Thank you for your question. The conjugation ratio to protein for our most recent lot is 12 PC/KLH (Phosphorylcholine hapten is conjugated to KLH protein by use of p-diazonium phenylphosphorylcholine). Unfortunately our synthesis methods are proprietary.
Answered by: Tech Service
Date published: 2019-04-09
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Rated 5 out of 5 by from 很好用啊之前买了这个产品，其实用量还挺大的，但是第一次抱着试试看的态度买了一只。使用过后，想再买的，据说不让进口了，也只能作罢。
Date published: 2021-03-08
Rated 5 out of 5 by from 功能很出色实验室应该前面买过该类产品，但已经用完了，所以在前不久，因科研需要，刚买的PC-KLH此产品,效果明显，功能出色，很好用
Date published: 2019-03-30
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PC-KLH (Keyhole Limpet Hemocyanin) is rated 5.0 out of 5 by 2.
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