Fig 1.
Map of Pearl River basin, Liujong River and Min River in southern China.
Distribution areas of botiid species indicated as follows: Green: S. pulchra, violet: joint distribution area of L. guilinensis and ‘B.’ zebra, pink: new record of ‘B.’ zebra. Circles with numbers indicate geographical origin of analysed samples. Numbers correspond to locality numbers in Table 1.
Table 1.
Number of specimens and geographical origin of Leptobotia guilinensis, ‘B.’ zebra and Sinibotia pulchra analysed in the present study.
Table 2.
Morphometric comparison of Leptobotia guilinensis, Sinibotia pulchra and ‘Botia’ zebra.
Fig 2.
Habitus of Leptobotia guilinensis, Sinibotia pulchra and ’B.’ zebra.
Specimens of (a) Leptobotia guilinensis, (b) ‘Botia’ zebra and (c) Sinibotia pulchra in lateral and dorsal view. ‘Botia’ zebra shares the general body shape and pigmentation pattern, the smaller head size and the shorter, round fins with L. guilinensis, but the pigmentation of the head with Sinibotia pulchra.
Fig 3.
Suborbital spines of Leptobotia guilinensis, Sinibotia pulchra and ’B.’ zebra.
Right suborbital spine of (a) Sinibotia pulchra (A1783, 70.5 mm SL), (b) ‘Botia’ zebra (A8607, 61.9 mm SL) and (c) Leptobotia guilinensis (A8573, 70.6 mm SL) in dorsal view. The spine bears a dorsal branch in S. pulchra, but this branch is missing in L. guilinensis and ‘B.’ zebra. A simple (= unbranched) suborbital spine is the diagnostic character for the genus Leptobotia. Scale bar is 1 mm.
Fig 4.
Mouthes of Leptobotia guilinensis, Sinibotia pulchra and ’B.’ zebra.
Mouth of a) Sinibotia pulchra, A 9102, 73.0 mm SL; b) ‘B.’ zebra, A 8904, 63.1 mm SL and c) Leptobotia guilinensis, A8868, 71.6 mm SL in ventral view. The presence of two prominent buttons on the lower lip (arrows in S. pulchra) are a diagnostic character of the genus Sinibotia. Scale bar is 1 mm.
Fig 5.
Karyotypes of Leptobotia guilinensis, Sinibotia pulchra and ’B.’ zebra.
Karyotypes of the diploid species Leptobotia guilinensis (a) and the tetraploid species ‘Botia’ zebra (b) and Sinibotia pulchra (c). Bar = 10 μm.
Table 3.
Chromosme numbers and karyotype composition of Leptobotia guilinensis, Sinibotia pulchra and ’B.’ zebra.
Table 4.
Allozymes of Leptobotia guilinensis, Sinibotia pulchra and ’B.’ zebra.
Table 5.
Specimens of Botiidae used in the DNA analyses.
Fig 6.
Phylogenetic relationships of Botiidae as revealed by the Cytochrome b dataset.
Phylogenetic relationships among freshwater fishes of the family Botiidae as revealed by a Bayesian analyses of the mitochondrial cytochrome b gene. The values at the nodes represent the Bayesian posterior probabilities. Sinibotia zebra and Sinibotia pulchra are sister species, while Leptobotia guilinensis is only distantly related.
Fig 7.
Phylogenetic relationships of Botiidae as revealed by the RAG-1 dataset.
Phylogenetic relationships among freshwater fishes of the family Botiidae as revealed by a Bayesian analyses of the nuclear RAG-1 gene. The values at the nodes represent the Bayesian posterior probabilities. Sinibotia zebra and Sinibotia pulchra are sister species, while Leptobotia guilinensis is only distantly related.